CYP4F2 Antibody (OAAF02964)

Catalog No: OAAF02964

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma, using Cytochrome P450 4F2 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human CYP4F2.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: Cytochrome P450 4F2 antibody detects endogenous levels of total Cytochrome P450 4F2 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; ELISA: 1:40000
• Reference: Kikuta Y., FEBS Lett. 348:70-74(1994).
  Grimwood J., Nature 428:529-535(2004).
  Zhang X., Arch. Biochem. Biophys. 378:364-376(2000).

Target Info

• Gene Symbol: CYP4F2
• Gene Full Name: cytochrome P450 family 4 subfamily F member 2
• Alias Symbols: 20-HETE synthase;20-hydroxyeicosatetraenoic acid synthase;arachidonic acid omega-hydroxylase;CPF2;CYPIVF2;cytochrome P450 4F2;cytochrome P450, family 4, subfamily F, polypeptide 2;cytochrome P450, subfamily IVF, polypeptide 2;cytochrome P450-LTB-omega;docosahexaenoic acid omega-hydroxylase;leukotriene-B(4) 20-monooxygenase 1;leukotriene-B(4) omega-hydroxylase 1;phylloquinone omega-hydroxylase CYP4F2.
• NCBI Gene Id: 8529
• Protein Name: Phylloquinone omega-hydroxylase CYP4F2
• Description of Target: Omega-hydroxylase that oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids and xenobiotics. Plays a key role in vitamin K catabolism by mediating omega-hydroxylation of vitamin K1 (phylloquinone), and menaquinone-4 (MK-4), a form of vitamin K2. Hydroxylation of phylloquinone and MK-4 probably regulates blood coagulation (PubMed:19297519, PubMed:24138531). Also shows arachidonic acid omega-hydroxylase activity in kidney, by mediating conversion of arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE), possibly influencing blood pressure control (PubMed:10660572, PubMed:17341693, PubMed:18574070). Also acts as a leukotriene-B(4) omega-hydroxylase by mediating conversion of leukotriene-B(4) (LTB4) to its omega-hydroxylated metabolite 20-hydroxyleukotriene-B(4) (20-OH LTB4) (PubMed:8026587, PubMed:9799565).
• Uniprot ID: P78329
• Protein Accession #: NP_001073.3
• Molecular Weight: 59 kDa