CYP4F2 ELISA Kit (Human) (OKEH01660)

Catalog No: OKEH01660

Size:96T

Human CYP4F2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CYP4F2 ELISA Kit (Human) (OKEH01660) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human CYP4F2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CYP4F2 and incubated. The CYP4F2 found in the sample or standards competes with the biotinylated CYP4F2 for limited binding sites on the immobilized anti-human CYP4F2 antibody. Excess unbound biotinylated CYP4F2 and sample or standard CYP4F2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CYP4F2 captured in the well and inversely proportional to the amount of CYP4F2 which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 7.8% (n = 20)
  Mean Inter-assay CV%: < 9.6% (n = 20)
• ELISA Kit Component:
  • Anti-CYP4F2 Microplate: 96 Wells (12 x 8 Well strips)
  • CYP4F2 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X CYP4F2-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.176 ng/mL
• Specificity: Natural and recombinant Human Phylloquinone omega-hydroxylase CYP4F2
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: CYP4F2
• Gene Full Name: cytochrome P450 family 4 subfamily F member 2
• Alias Symbols: 20-HETE synthase;20-hydroxyeicosatetraenoic acid synthase;arachidonic acid omega-hydroxylase;CPF2;CYPIVF2;cytochrome P450 4F2;cytochrome P450, family 4, subfamily F, polypeptide 2;cytochrome P450, subfamily IVF, polypeptide 2;cytochrome P450-LTB-omega;docosahexaenoic acid omega-hydroxylase;leukotriene-B(4) 20-monooxygenase 1;leukotriene-B(4) omega-hydroxylase 1;phylloquinone omega-hydroxylase CYP4F2.
• NCBI Gene Id: 8529
• Protein Name: P78329
• Description of Target: Phylloquinone omega-hydroxylase CYP4F2
• Uniprot ID: https://www.uniprot.org/uniprot/P78329
• Protein Accession #: NP_001073.3
• Nucleotide Accession #: NM_001082.4
• Protein Size (# AA): 520
• Molecular Weight: 59 kDa