CYP24A1 Antibody (OAGA00442)

Catalog No: OAGA00442

Size:100 ul

Sample(30?g whole cell lysate)
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
F: MOLT4
G: Raji
7.5% SDS PAGE
OAGA00442 diluted at 1:3000

CYP24A1 antibody detects CYP24A1 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
CYP24A1 antibody (OAGA00442) dilution: 1:500.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
  Positive Controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji
  Cellular Localization: Mitochondrion
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 241 and 514 of CYP24A1 (Uniprot ID#Q07973)
• Purification: Purified by antigen-affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse: 87%; Rat: 88%
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows:
  WB: 1:500-1:3000,
  IHC-FFPE: Optimal working dilutions should be determined experimentally by the end user.
  Not yet tested in other applications.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: CYP24A1
• Gene Full Name: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
• Alias Symbols: CP24, HCAI, CYP24, HCINF1, P450-CC24
• NCBI Gene Id: 1591
• Protein Name: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
• Description of Target: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Uniprot ID: Q07973
• Protein Accession #: NP_000773.2
• Molecular Weight: 59 kDa