CYP24A1 Antibody (OABB02030)

Catalog No: OABB02030

Size:100UG

Anti-CYP24A1 Picoband antibody, OABB02030, Western blotting
All lanes: Anti CYP24A1 (OABB02030) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: 22RV1 Whole Cell Lysate at 40ug
Lane 5: A431 Whole Cell Lysate at 40ug
Lane 6: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 59KD
Observed bind size: 59KD

Anti-CYP24A1 Picoband antibody, OABB02030, IHC(P)
IHC(P): Human Pancreatic Cancer Tissue

Anti-CYP24A1 Picoband antibody, OABB02030, IHC(P)
IHC(P): Mouse Kidney Tissue

Anti-CYP24A1 Picoband antibody, OABB02030, IHC(P)
IHC(P): Rat Kidney Tissue

Product Info

• Publications:

  Hiroki Maruyama, et al. Low bone mineral density due to secondary hyperparathyroidism in the GlatmTg(CAG-A4GALT) mouse model of Fabry disease. 365-381 (2020). 32617522

  More...

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Lyophilized Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: Reconstitute in 0.2 ml of distilled water to produce a concentration of 500 ug/mL. Store at -20°C for one year from date of receipt. After reconstitution, store at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze/thaw cycles.
• Immunogen: E.coli-derived human CYP24A1 recombinant protein (Position: E153-R514). Human CYP24A1 shares 86.2% amino acid (aa) sequence identity with both mouse and rat CYP24A1.
• Purification: Affinity Purified
• Specificity: No cross reactivity with other proteins.
• Application Info: WB: 0.1-0.5 ug/mL; Human, Rat, Mouse
  IHC-P: 0.5-1 ug/mL; Human, Rat, Mouse
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Target Info

• Reference: 1. "Entrez Gene: CYP24A1 cytochrome P450, family 24, subfamily A, polypeptide 1".
  2. Dauber A, Nguyen TT, Sochett E, Cole DE, Horst R, Abrams SA, Carpenter TO, Hirschhorn JN (February 2012). "Genetic defect in CYP24A1, the vitamin D 24-hydroxylase gene, in a patient with severe infantile hypercalcemia". J. Clin. Endocrinol. Metab. 97 (2): E268-74.
  3. Sakaki T, Kagawa N, Yamamoto K, Inouye K (January 2005). "Metabolism of vitamin D3 by cytochromes P450". Front. Biosci. 10: 119-34.
  
• Gene Symbol: CYP24A1
• Gene Full Name: cytochrome P450 family 24 subfamily A member 1
• Alias Symbols: 1,25-@dihydroxyvitamin D3 24-hydroxylase;1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial;24-OHase;CP24;CYP24;cytochrome P450 24A1;cytochrome P450, family 24, subfamily A, polypeptide 1;cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase);cytochrome P450-CC24;exo-mitochondrial protein;HCAI;HCINF1;P450-CC24;vitamin D 24-hydroxylase;vitamin D(3) 24-hydroxylase.
• NCBI Gene Id: 1591
• Protein Name: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
• Description of Target: Has a role in maintaining calcium homeostasis. Catalyzes the adrenodoxin-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23-hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product.
• Uniprot ID: Q07973
• Molecular Weight: 59 kDa