CTSD High Sensitivity ELISA Kit (Human) (OKCD01351)

Catalog No: OKCD01351

Size:96WELLS

Human CTSD ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	88-102%	78-104%	78-101%	81-97%
  EDTA Plasma (n=5)	89-98%	91-101%	80-92%	81-94%
  Heparin Plasma (n=5)	91-105%	92-105%	79-102%	82-92%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Cathepsin D (CTSD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Cathepsin D (CTSD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Cathepsin D (CTSD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Cathepsin D (CTSD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 46.88-3,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	91-98	94
  EDTA Plasma (n=5)	85-105	93
  Heparin Plasma (n=5)	82-101	89

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Cathepsin D (CTSD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Cathepsin D (CTSD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CTSD Microplate: 96 Wells (12 x 8 Well Strips)
  • CTSD Lyophilized Standard: 2
  • 100X Biotinylated CTSD Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 15.27 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cathepsin D (CTSD). No significant cross-reactivity or interference between Cathepsin D (CTSD) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CTSD
• Gene Full Name: cathepsin D
• Alias Symbols: cathepsin D;ceroid-lipofuscinosis, neuronal 10;CLN10;CPSD;epididymis secretory sperm binding protein Li 130P;HEL-S-130P;lysosomal aspartyl peptidase;lysosomal aspartyl protease.
• NCBI Gene Id: 1509
• Protein Name: Cathepsin D
• Description of Target: Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation (PubMed:27333034). Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
• Uniprot ID: P07339
• Protein Accession #: NP_001900.1
• Nucleotide Accession #: NM_001909.4