Cathepsin D ELISA Kit (Mouse) (OKBB00636)

Catalog No: OKBB00636

Size:96 Wells

Mouse Cathepsin D ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse Cathepsin D ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for Cathepsin D has been precoated onto 96-well plates. Standards(NS0, I21-L410) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Cathepsin D amount of sample captured in plate.
• ELISA Kit Range: 312 pg/ml - 20,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	1.13	3.29	6.68	1.19	3.34	6.73
  SD	0.033	0.115	0.247	0.043	0.134	0.283
  CV%	2.9	3.5	3.7	3.6	4	4.2

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse Cathepsin D Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse Cathepsin D Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse Cathepsin D Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse Cathepsin D
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: I21-L410
• Description: For quantitative detection of mouse Cathepsin D in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: CTSD
• Gene Full Name: cathepsin D
• Alias Symbols: CATD_HUMAN, Cathepsin D heavy chain, Ceroid lipofuscinosis neuronal 10, CLN10, CPSD, ctsd, Lysosomal aspartyl protease
• NCBI Gene Id: 13033
• Protein Name: Cathepsin D
• Description of Target: Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
• Uniprot ID: P18242
• Protein Accession #: NP_034113.1
• Nucleotide Accession #: NM_009983.2