CTCF Antibody - N-terminal region (P100757_P050)

Catalog No: P100757_P050

WB Suggested Anti-CTCF Antibody Titration: 0.2-1 ug/ml
Positive Control: Transfected 293T

Chromatin Immunoprecipitation (ChIP) Using CTCF antibody - N-terminal region (P100757_P050) and HCT116 Cells

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: CHIP, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CTCF
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: GELPPQEDPSWQKDPDYQPPAKKTKKTKKSKLRYTEEGKDVDVSVYDFEE
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-CTCF (P100757_P050) antibody is Catalog # AAP31079 (Previous Catalog # AAPP01818)
• Reference: Defossez,P.A., (2005) J. Biol. Chem. 280 (52), 43017-43023

Target Info

• Gene Symbol: CTCF
• Gene Full Name: CCCTC-binding factor (zinc finger protein)
• Alias Symbols: MRD21, FAP108, CFAP108
• NCBI Gene Id: 10664
• Protein Name: Transcriptional repressor CTCF
• Description of Target: CTCF is a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in CTCF have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.
• Uniprot ID: P49711
• Protein Accession #: NP_006556
• Nucleotide Accession #: NM_006565
• Protein Size (# AA): 727
• Molecular Weight: 83kDa
• Protein Interactions: ZMYM4; UBC; EED; BMI1; KANSL1; CDK19; ZMYM2; CAND1; ZBTB33; TFAP4; SUMO1; SUMO2; SUMO3; SIRT7; TAF3; SMC3; POLR2A; SUZ12; Ctcf; HIST2H2AC; TOP2A; TAF1; NPM1; LMNA; KPNA4; KPNA2; H2AFZ; PARP1; TRB; RXRA; SMAD3; SIN3A; SMC1A; RAD21; YY1; THRB; SMAD4; HMGB1;