CTCF Antibody (OAAD00392)

Catalog No: OAAD00392

Size:0.1ml

western blot analysis using CTCF mouse mAb against A31 (1), MCF-7 (2), Hela (3), HCT116 (4), Jurkat (5), NIH/3T3 (6), and Cos7 (7) cell lysate.

Product Info

• Predicted Species Reactivity: Human, monkey
• Clonality: Monoclonal
• Clone: 1D11
• Isotype: IgG1
• Host: Mouse
• Application: WB
• Reconstitution and Storage: Store at 4C, for long term storage, store at -20C.
• Immunogen: Purified recombinant fragment of human CTCF expressed in E. Coli.
• Formulation: Ascitic fluid containing 0.03% sodium azide.
• Application Info: Western Bloting: 1/500 - 1/2000.
  ELISA: Propose dilution 1/10000.
  Not yet tested in other applications.
  Determining optimal working dilutions by titration test.
• Reference: 1. J Mol Med. 2008 Sep;86(9):1057-66.
  2. J Exp Med. 2008 Apr 14;205(4):785-98.
  3. EMBO J. 2008 Feb 20;27(4):654-66.

Target Info

• Alias Symbols: MRD21, FAP108, CFAP108
• NCBI Gene Id: 10664
• Description of Target: This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)- containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.Tissue specificity: Ubiquitous.
  Absent in primary spermatocytes.
• Uniprot ID: P49711
• Molecular Weight: 83kDa