CFP Chemi-Luminescent ELISA Kit (Human) (OKCD03372)

Catalog No: OKCD03372

Size:96WELLS

Human CFP ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	96-105%	82-90%	88-102%	80-89%
  EDTA Plasma (n=5)	89-97%	87-99%	94-101%	80-91%
  Heparin Plasma (n=5)	87-99%	97-105%	79-102%	91-99%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Complement Factor P (CFP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Complement Factor P (CFP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement Factor P (CFP) level in the sample or standard.
• ELISA Kit Range: 0.27-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	89-103	96
  EDTA Plasma (n=5)	86-97	93
  Heparin Plasma (n=5)	97-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor P (CFP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor P (CFP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CFP Microplate: 96 Wells (12 x 8 Well strips)
  • CFP Lyophilized Standard: 2
  • 100X CFP Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.27 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CFP
• Gene Full Name: complement factor properdin
• Alias Symbols: BFD;complement factor P;PFC;PFD;properdin;properdin P factor, complement.
• NCBI Gene Id: 5199
• Protein Name: Properdin
• Description of Target: A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes.
• Uniprot ID: P27918
• Protein Accession #: NP_001138724
• Nucleotide Accession #: NM_001145252