Catalog No: OKEH03282
Size:96 Wells
Price: $525.00
SKU
OKEH03282
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CFP ELISA Kit (Mouse) (OKEH03282) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cfp has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Cfp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cfp captured in well.
ELISA Kit Range15.6 - 1000pg/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.4% (n = 20)
Mean Inter-assay CV%: <= 8.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Cfp Microplate96 Wells (12 x 8 Well strips)
Cfp Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Cfp Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity7.8 pg/mL
SpecificityNatural and recombinant Mouse Properdin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCFP
Gene Full Namecomplement factor properdin
Alias SymbolsPf, Pfc, BCFG
NCBI Gene Id18636
Protein NameProperdin
Description of TargetA positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes.
Swissprot IdP11680
Protein Accession #NP_032849.2
Nucleotide Accession #NM_008823.4
Protein Size (# AA)464
Molecular Weight50 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express Cfp.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express Cfp.
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