CD80 Antibody (OAEC04503)

Catalog No: OAEC04503

Size:100 ug/100 ul

Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-CD80 mAb

Confocal immunofluorescence analysis of BCBL-1 cells using anti-CD80 monoclonal antioby (green), showing membrane localization. Blue. DRAQ5 fluorescent DNA dye.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Ascitic fluid containing 0.03% sodium azide
• Clonality: Monoclonal
• Clone: 2A2
• Isotype: IgG1
• Host: Mouse
• Application: IF, IHC
• Reconstitution and Storage: Store at 4C, for long term storage, store at -20C.
• Immunogen: Purified recombinant fragment of CD80 expres sed in EColi.
• Concentration: 1 mg/ml
• Application Info: IHC: 1/200 - 1/1000
  IF: 1/200 - 1/1000
  ELISA: 1/10000
  Figure 1 Immunohistochemical analysis of paraffin-embedded human Tonsil tissues usinganti-CD80 mAb

Target Info

• Gene Symbol: CD80
• Gene Full Name: CD80 molecule
• Alias Symbols: B7, BB1, B7-1, B7.1, LAB7, CD28LG, CD28LG1
• NCBI Gene Id: 941
• Description of Target: The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. Its principal mode of action is by binding to CD28. Along with CD86, these molecules provide the necessary stimuli to prime T cells against antigens presented by antigen-presenting cells. CD80 and CD86 also bind to CTLA-4, a cell surface molecule expressed on activated T cells. Interactions between CD80 or CD86 with CTLA-4 decrease the response of T cells. Mouse research by scientists at Emory University showed that estrogen-related bone loss is linked to recently discovered pathways involving various proteins, such as CD80 and other functions. In a nutshell, reactive oxygen stimulates dendritic cells, which activate other immune cells to up-regulate production of CD80, the molecule co-responsible for T cell activation. "When this pathway is activated, it leads to increased T cell TNF production and ultimately to bone loss." In turn, T cells produce a protein, Tumor Necrosis Factor, which increases the formation of osteoclasts in rodents and humans.
• Uniprot ID: P33681
• Molecular Weight: 55 kDa