CD48 Antibody (OAEE00899)

Catalog No: OAEE00899

Size:100T

Surface staining of human peripheral blood cells with anti-CD48 (MEM-102) Alexa Fluor 700.

Product Info

• Predicted Species Reactivity: Human|Non-human Primate
• Product Format: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: MEM-102
• Isotype: IgG1
• Host: Mouse
• Conjugation: R-PE
• Application: Flow cytometry
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Raji human Burkitt's lymphoma cell line
• Purification: Purified by size-exclusion chromatography
• Concentration: 20 ul / test
• Specificity: The antibody MEM-102 reacts with CD48 antigen (Blast-1), a 40-47 kDa GPI-anchored membrane protein (immunoglobulin supergene family) widely expressed on hematopoietic cells; it is negative on granulocytes, platelets and erythrocytes.
  HLDA V; WS Code AS S014
• Formulation: PE
• Storage Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
• Reference: Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Bukovsky A, Horejsi V: Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha). 1989;35(5):289-97.
  Stulnig TM, Berger M, Sigmund T, Stockinger H, Horejsí V, Waldhäusl W: Signal transduction via glycosyl phosphatidylinositol-anchored proteins in Tcells is inhibited by lowering cellular cholesterol. J Biol Chem. 1997 Aug 1;272(31):19242-7.
  Angelisová P, Drbal K, Horejsí V, Cerný J: Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase. Blood. 1999 Feb 15;93(4):1437-9.
  Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84.
  Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., Korinek V, Stefanova I, Angelisova P, Hilgert I, Horejsi V: The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1. Immunogenetics. 1991;33(2):108-12.
  Munitz A, Bachelet I, Eliashar R, Khodoun M, Finkelman FD, Rothenberg ME, Levi-Schaffer F: CD48 is an allergen and IL-3-induced activation molecule on eosinophils. J Immunol. 2006 Jul 1;177(1):77-83.
  Lee KM, Forman JP, McNerney ME, Stepp S, Kuppireddi S, Guzior D, Latchman YE, Sayegh MH, Yagita H, Park CK, Oh SB, Wülfing C, Schatzle J, Mathew PA, Sharpe AH,Kumar V: Requirement of homotypic NK-cell interactions through 2B4(CD244)/CD48 in the generation of NK effector functions. Blood. 2006 Apr 15;107(8):3181-8.
  Mathew SO, Kumaresan PR, Lee JK, Huynh VT, Mathew PA: Mutational analysis of the human 2B4 (CD244)/CD48 interaction: Lys68 and Glu70 in the V domain of 2B4 are critical for CD48 binding and functional activation of NKcells. J Immunol. 2005 Jul 15;175(2):1005-13.
  Assarsson E, Kambayashi T, Persson CM, Chambers BJ, Ljunggren HG: 2B4/CD48-mediated regulation of lymphocyte activation and function. J Immunol. 2005 Aug 15;175(4):2045-9.

Target Info

• Gene Symbol: CD48
• Gene Full Name: CD48 molecule
• Alias Symbols: BCM1;BCM1 surface antigen;BLAST;BLAST1;B-lymphocyte activation marker BLAST-1;CD48 antigen;CD48 antigen (B-cell membrane protein);hCD48;leukocyte antigen MEM-102;mCD48;MEM-102;signaling lymphocytic activation molecule 2;SLAM family member 2;SLAMF2;TCT.1.
• NCBI Gene Id: 962
• Protein Name: CD48 antigen
• Description of Target: Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.
• Uniprot ID: P09326
• Protein Accession #: NP_001769.2
• Nucleotide Accession #: NM_001778.3