CBFB Antibody (OADC00013)

Catalog No: OADC00013

Size:100ul

ChIP results obtained with the Aviva antibody directed against CBFb. ChIP assays were performed using SKNO-1 cells, the Aviva antibody against CBFb and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, OGG1, NFE2, and SPI1 genes. Figure shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.

ChIP-seq results obtained with the Aviva antibody directed against CBFb r. ChIP was performed as described above. The IP'd DNA from 6 ChIP's was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure shows the results of the complete chromosome 3 and three genomic regions region surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.

Determination of the antibody titer. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Aviva antibody directed against human CBFb. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution the titer of the antibody was estimated to be 1:8,800.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Whole antiserum from rabbit containing 0.05% azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: CHIP
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Purification: Whole antiserum
• Concentration: Undetermined, ascites
• Preservative: 0.05% Sodium Azide
• Specificity: Polyclonal antibody raised in rabbit against human CBFb (core-binding factor, beta subunit) using two KLH-conjugated synthetic peptides containing sequences from the central region of the protein.
• Application Info: CHIP: 4 ul/CHIP
  ELISA: 1:500

Target Info

• Gene Symbol: CBFB
• Gene Full Name: core-binding factor, beta subunit
• Alias Symbols: PEBP2B
• NCBI Gene Id: 865;
• Protein Name: core-binding factor subunit beta
• Description of Target: The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: Q13951
• Protein Accession #: NP_001746.1
• Nucleotide Accession #: NM_001755.2