CBFB Antibody (OADC00013)

• Product Number: OADC00013
• Name: CBFB Antibody (OADC00013)
• Conjugation: Unconjugated
• NCBI Gene Id: 865;
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Undetermined, ascites
• Gene Full Name: core-binding factor, beta subunit
• Alias Symbols: PEBP2B
• Product Format: Liquid. Whole antiserum from rabbit containing 0.05% azide.
• Description of Target: The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene.
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Specificity: Polyclonal antibody raised in rabbit against human CBFb (core-binding factor, beta subunit) using two KLH-conjugated synthetic peptides containing sequences from the central region of the protein.
• Application Info: CHIP: 4 ul/CHIP
  ELISA: 1:500
• Uniprot ID: Q13951
• Protein Name: core-binding factor subunit beta
• Protein Accession #: NP_001746.1
• Purification: Whole antiserum
• Nucleotide Accession #: NM_001755.2
• Preservative: 0.05% Sodium Azide
• Gene Symbol: CBFB
• Predicted Species Reactivity: Human
• Application: CHIP
• Image 1: SKNO-1 cells
  
  ChIP results obtained with the Aviva antibody directed against CBFb. ChIP assays were performed using SKNO-1 cells, the Aviva antibody against CBFb and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, OGG1, NFE2, and SPI1 genes. Figure shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.
• Image 2: Human reference genome (hg18)
  
  ChIP-seq results obtained with the Aviva antibody directed against CBFb r. ChIP was performed as described above. The IP'd DNA from 6 ChIP's was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure shows the results of the complete chromosome 3 and three genomic regions region surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.
• Image 3: Human CBFb
  
  Determination of the antibody titer. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Aviva antibody directed against human CBFb. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution the titer of the antibody was estimated to be 1:8,800.