CBFB Antibody - N-terminal region (OASG05780)

Catalog No: OASG05780

Size:100 uL

Western Blot using HUVEC

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC, IF, ELISA
• Additional Information: Cellular Localization: Nucleus.
• Reconstitution and Storage: Store at -20°C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from the N-terminal region of human PEBP2Beta. Location: 30-110aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Specificity: PEBP2Beta Polyclonal Antibody detects endogenous levels of PEBP2Beta protein.
• Application Info: WB 1:500~2000
  IHC 1:100~300
  IF 1:200~1000
  ELISA 1:20000
  

Target Info

• Gene Symbol: CBFB
• Gene Full Name: core-binding factor, beta subunit
• Alias Symbols: PEBP2B
• NCBI Gene Id: 865; 12400;
• Description of Target: Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation.
• Uniprot ID: Q13951, Q08024
• Molecular Weight: 22 kDa