CASP3 ELISA Kit (Rat) (OKCA00955)

Catalog No: OKCA00955

Size:96 Wells

Rat CASP3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	84	80-92
  1:2	98	91-105
  1:4	99	92-110
  1:8	91	86-98

• ELISA Kit Principle: Aviva Systems Biology CASP3 ELISA Kit (Rat) (OKCA00955) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for CASP3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CASP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CASP3 captured in well.
• ELISA Kit Range: 0.312 ng/mL-20 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	95	89-98
  EDTA Plasma (n=4)	96	90-100

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level CASP3 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level CASP3 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • CASP3 Microplate: 96 Wells (12 x 8 Well strips)
  • CASP3 Lyophilized Standard: 2
  • 100X Biotinylated CASP3 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.078 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CASP3
• Gene Full Name: Caspase-3
• Alias Symbols: Apopain, CASP-3, Caspase-3, Cysteine protease CPP32
• NCBI Gene Id: 100008840
• Protein Name: Caspase-3
• Description of Target: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage.
• Uniprot ID: Q8MJC3
• Protein Accession #: NP_001075586.1
• Nucleotide Accession #: NM_001082117.1