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- $55: Antibody & Protein in US
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| Datasheets/Manuals | Printable datasheet for CASP3 (Cleaved-Asp175) Antibody (OAAF05314) |
|---|
| Predicted Species Reactivity | Human|Mouse|Rat |
|---|---|
| Product Format | Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot |
| Reconstitution and Storage | -20°C |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Caspase 3. |
| Purification | The antibody was purified from rabbit antiserum by affinity chromatography using immunogen. |
| Concentration | 1 mg/ml |
| Specificity | Caspase 3 (Cleaved-Asp175) Antibody detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175. |
| Application Info | WB: 1:500~1:1,000 IHC: 1:50~1:100 ELISA: 1:20,000 Optimal dilutions should be determined by the end user. |
| Reference | John K. Jain, Biol Reprod, Aug 2005; 73: 382 - 388. C.S. Thota, Biol Reprod, Dec 2005; 73: 1191 - 1198. Koichi Ushizawa, Biol Reprod, Feb 2006; 74: 344 - 351. ULRICH STEIDL, Ann. N.Y. Acad. Sci., Jun 2005; 1044: 94 - 108. |
| Gene Symbol | CASP3 |
|---|---|
| Gene Full Name | caspase 3 |
| Alias Symbols | apopain;CASP-3;caspase 3, apoptosis-related cysteine peptidase;caspase 3, apoptosis-related cysteine protease;caspase-3;CPP32;CPP-32;CPP32B;cysteine protease CPP32;PARP cleavage protease;procaspase3;protein Yama;SCA-1;SREBP cleavage activity 1. |
| NCBI Gene Id | 836 |
| Protein Name | Caspase-3 |
| Description of Target | Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. |
| Uniprot ID | P42574 |
| Protein Accession # | NP_004337.2 |
| Molecular Weight | 20 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CASP3 (Cleaved-Asp175) Antibody (OAAF05314)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "CASP3 (Cleaved-Asp175) Antibody (OAAF05314)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "CASP3 (Cleaved-Asp175) Antibody (OAAF05314)" provided in?
This item is provided in "Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CASP3 (Cleaved-Asp175) Antibody (OAAF05314)"?
This target may also be called "apopain;CASP-3;caspase 3, apoptosis-related cysteine peptidase;caspase 3, apoptosis-related cysteine protease;caspase-3;CPP32;CPP-32;CPP32B;cysteine protease CPP32;PARP cleavage protease;procaspase3;protein Yama;SCA-1;SREBP cleavage activity 1." in publications.
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What is the shipping cost for "CASP3 (Cleaved-Asp175) Antibody (OAAF05314)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CASP3 (Cleaved-Asp175) Antibody (OAAF05314)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CASP3 (Cleaved-Asp175) Antibody (OAAF05314)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "20 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CASP3 (Cleaved-Asp175) Antibody (OAAF05314)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CASP3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CASP3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CASP3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CASP3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CASP3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CASP3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
