CASP3 Antibody - N-terminal region (AVARP00021_T100)

Catalog No: AVARP00021_T100

WB Suggested Anti-CASP3 Antibody Titration: 5ug/ml
Positive Control: Human Liver

Immunofluorescent Caspase 3 detection in HeLa cells after induction of apoptosis (red fluorescence). Nuclei were stained with DAPI (blue fluorescence).

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CASP3
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 84%; Dog: 84%; Guinea Pig: 84%; Horse: 92%; Human: 100%; Pig: 92%; Rabbit: 84%; Rat: 84%
• Peptide Sequence: Synthetic peptide located within the following region: MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIII
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-CASP3 (AVARP00021_T100) antibody is Catalog # AAP30492 (Previous Catalog # AAPP01128)
• Reference: Selvakumar,P., et al., (2006) FEBS Lett. 580 (8), 2021-2026
• Publications:

  A scaffold-free surface culture of B16F10 murine melanoma cells based on magnetic levitation. Cytotechnology. 68, 2323-2334 (2016). 27670438

Target Info

• Gene Symbol: CASP3
• Gene Full Name: Caspase 3, apoptosis-related cysteine peptidase
• Alias Symbols: CPP32, SCA-1, CPP32B
• NCBI Gene Id: 836
• Protein Name: Caspase-3
• Description of Target: CASP3 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
• Uniprot ID: P42574
• Protein Accession #: NP_004337
• Nucleotide Accession #: NM_004346
• Protein Size (# AA): 277
• Molecular Weight: 32 kDa
• Protein Interactions: BAX; PARP1; PTGES3; XIAP; CRYAB; BIRC7; UBC; MDM2; Gzmb; VIM; STAT1; SPTAN1; ROCK1; GSN; DFFA; CASP9; CASP7; CASP6; CASP2; BID; ARHGDIA; TNFSF10; PLA2G4B; CDKN1A; RB1; PRKDC; GLRX; GATA1; SOCS5; YWHAG; MAPK9; MAPK8; SP1; PKN1; CASP8; BIRC3; BIRC2; ZBTB16;