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C-Peptide ELISA Kit (Rat) (OKEH03700)

96 Wells
$529.00
In Stock
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Gene Symbol:
C-PEPTIDE
Alias Symbols:
Proinsulin C-peptide, 59112-80-0, AM026425
Description of Target:
C-peptide is a protein that is produced in the body along with insulin. First preproinsulin is secreted with an A-chain, C-peptide, a B-chain, and a signal sequence. The signal sequence is cut off, leaving proinsulin. Then the C-peptide is cut off, leaving the A-chain and B-chain to form insulin.
Predicted Species Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 39 pg/mL
Kit Range:
78 - 5,000 pg/mL
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology C-Peptide ELISA Kit (Rat) (OKEH03700) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human C-Peptide antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated C-Peptide and incubated. The C-Peptide found in the sample or standards competes with the biotinylated C-Peptide for limited binding sites on the immobilized anti-human C-Peptide antibody. Excess unbound biotinylated C-Peptide and sample or standard C-Peptide is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated C-Peptide captured in the well and inversely proportional to the amount of C-Peptide which was contained in the sample or standard.
Kit Component:
ComponentAmount
Anti-C-Peptide Microplate96 Wells (12 x 8 Well strips)
C-Peptide Lyophilized Standard2
Sample Diluent1 x 20 mL
100X C-Peptide-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural Rat C-Peptide. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Competitive Immunoassay
Additional Information:

Molecular Formula: C112H179N35O46

CAS Number: 59112-80-0

PubChem: 16132309

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