Catalog No: OKEH00454
Price: $525.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology C-Peptide ELISA Kit (Mouse) (OKEH00454) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse C-Peptide antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Mouse C-Peptide and incubated. The Mouse C-Peptide found in the sample or standards competes with the biotinylated Mouse C-Peptide for limited binding sites on the immobilized anti-Mouse C-Peptide antibody. Excess unbound biotinylated Mouse C-Peptide and sample or standard Mouse C-Peptide is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Mouse C-Peptide captured in the well and inversely proportional to the amount of Mouse C-Peptide which was contained in the sample or standard.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 10.2% (n = 20)
ELISA Kit Component
Anti-C-Peptide Microplate96 Wells (12 x 8 Well strips)
C-Peptide Lyophilized Standard2
Sample Diluent1 x 20 mL
100X C-Peptide-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.083 ng/mL
SpecificityNatural and recombinant Mouse C-Peptide
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolC-PEPTIDE
Alias SymbolsC Peptide, C Peptide, Proinsulin,C-Peptide,C-Peptide, Proinsulin ,Connecting Peptide,Proinsulin C Peptide,Proinsulin C-Peptide
Protein NameC-Peptide
Description of TargetC-peptide is a protein that is produced in the body along with insulin. First preproinsulin is secreted with an A-chain, C-peptide, a B-chain, and a signal sequence. The signal sequence is cut off, leaving proinsulin. Then the C-peptide is cut off, leaving the A-chain and B-chain to form insulin.
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