C-Peptide ELISA Kit (Dog) (OKEH00455)

Catalog No: OKEH00455

Size:96T

Dog C-Peptide ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Dog
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology C-Peptide ELISA Kit (Dog) (OKEH00455) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Dog C-Peptide antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Dog C-Peptide and incubated. The Dog C-Peptide found in the sample or standards competes with the biotinylated Dog C-Peptide for limited binding sites on the immobilized anti-Dog C-Peptide antibody. Excess unbound biotinylated Dog C-Peptide and sample or standard Dog C-Peptide is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Dog C-Peptide captured in the well and inversely proportional to the amount of Dog C-Peptide which was contained in the sample or standard.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.3% (n = 20)
  Mean Inter-assay CV%: < 8.2% (n = 20)
• ELISA Kit Component:
  • Anti-C-Peptide Microplate: 96 Wells (12 x 8 Well strips)
  • C-Peptide Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X C-Peptide-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.069 ng/mL
• Specificity: Natural and recombinant Dog C-Peptide
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: C-PEPTIDE
• Alias Symbols: C Peptide, C Peptide, Proinsulin,C-Peptide,C-Peptide, Proinsulin ,Connecting Peptide,Proinsulin C Peptide,Proinsulin C-Peptide
• Protein Name: C-Peptide
• Description of Target: C-peptide is a protein that is produced in the body along with insulin. First preproinsulin is secreted with an A-chain, C-peptide, a B-chain, and a signal sequence. The signal sequence is cut off, leaving proinsulin. Then the C-peptide is cut off, leaving the A-chain and B-chain to form insulin.