Bax Antibody (BH3 Domain Specific) (OAAB09319)

Catalog No: OAAB09319

Size:400ul

Bax Antibody(BH3)(Cat. #OAAB09319) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Bax antibody detected the Bax protein (arrow).

Western blot analysis of Bax (arrow) using rabbit polyclonal Bax Antibody (BH3) (Cat. #OAAB09319). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bax gene.

BAX is supported by BioGPS gene expression data to be expressed in HL60

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with Bax Antibody (BH3 Domain Specific), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of HepG2 cells using Bax Antibody (BH3 Domain Specific)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Cow
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Additional Information: Precautions: Bax Antibody (BH3 Domain Specific) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis
  Cellular Location: Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14- 3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane Isoform Gamma: Cytoplasm.
  Tissue Location: Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro- myelocytic leukemia, histiocytic lymphoma, Burk
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This Bax antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-76 amino acids from human Bax.
• Predicted Homology Based on Immunogen Sequence: Cow; Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  BAX is supported by BioGPS gene expression data to be expressed in HL60

• Application Info: FC: 1:25
  WB: 1:2000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: BAX
• Alias Symbols: BCL2L4
• NCBI Gene Id: 581
• Protein Name: Apoptosis regulator BAX
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q07812
• Protein Accession #: NP_004315.1
• Molecular Weight: 21184 Da