ATG7 Antibody - C-terminal region (OAAB13623)

Catalog No: OAAB13623

Size:400ul

Western blot analysis of APG7L (C-term) Pab (Cat. #OAAB13623) in 293 cell line lysates transiently transfected with the ATG7 gene(2ug/lane).

Western blot analysis of APG7L Pab (Cat. #OAAB13623) in 293 cell line lysate (35ug/lane). APG7L (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy APG7L antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Fluorescent image of U251 cells stained with ATG7 (C-term) antibody. U251 cells were treated with Chloroquine (50 ?M, 16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with OAAB13623 ATG7 (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ?g/ml, 5 min). ATG7 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000197548).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IF, IHC-P
  Cellular Location: Cytoplasm. Preautophagosomal structure. Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
  Tissue Location: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-569 amino acids from the C-terminal region of human ATG7.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Chicken, Rat
• Concentration: 0.25 mg/ml
• Application Info: WB: 1:500
  WB: 1:1000
  IF: 1:25
  IHC-P: 1:50~100

Target Info

• Gene Symbol: ATG7
• Alias Symbols: GSA7, APG7L, APG7-LIKE
• NCBI Gene Id: 10533
• Protein Name: Ubiquitin-like modifier-activating enzyme ATG7
• Description of Target: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.
• Uniprot ID: O95352
• Protein Accession #: NP_001129503.2
• Molecular Weight: 78 kDa