ATG7 Antibody (OAPB00349)

Catalog No: OAPB00349

Size:100UG

Western blot analysis of APG7 in Caco-2 cell lysate with APG7 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.

Immunofluorescence of APG7 in MCF7 cells with APG7 antibody at 20 ug/mL.

Immunocytochemistry of APG7 in MCF7 cells with APG7 antibody at 10 μg/mL.

Product Info

• Predicted Species Reactivity: Chicken|Human|Mouse|Rat
• Product Format: Liquid. APG7 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: APG7 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: APG7 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human APG7. The immunogen is located within amino acids 590 - 640 of APG7.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-ATG7 (OAPB00349) antibody is Catalog # OEPB02439
• Application Info: APG7 antibody can be used for the detection of APG7 by Western blot at 0.5 - 1 ug/ml. (Optimal dilution should be determined by user)
• Reference: Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.;Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.;Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.;Mizushima N, Noda T, Yoshimori T, et al. A protein conjugation system essential for autophagy. Nature 1998; 395:395-8.

Target Info

• Gene Symbol: ATG7
• Gene Full Name: autophagy related 7
• Alias Symbols: APG7 autophagy 7-like;APG7L;APG7-LIKE;ATG12-activating enzyme E1 ATG7;Autophagy-related protein 7;GSA7;hAGP7;SCAR31;ubiquitin-activating enzyme E1-like protein;ubiquitin-like modifier-activating enzyme ATG7.
• NCBI Gene Id: 10533
• Protein Name: Ubiquitin-like modifier-activating enzyme ATG7|ubiquitin-like modifier-activating enzyme ATG7 isoform a [Homo sapiens]
• Description of Target: E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.
• Uniprot ID: O95352
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_006386
• Nucleotide Accession #: NM_001136031.2; NM_001144912.1; NM_006395.2
• Molecular Weight: Predicted: 69, 74, 77 kDa Observed: 77 kDa