Aquaporin 2 Antibody (Phospho-Ser261) (OAPC00158)

Catalog No: OAPC00158

Size:100 ul

Western blot of rat kidney lysate showing specific immunolabeling of the ~ 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser261. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide) but not by the corresponding dephospho-peptide (not shown).

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Bovine, Chicken, Canine, Non-human primate
• Product Format: Liquid.10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol.
  Adequate amount of material to conduct 10 mini Western blots
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, DB, IHC
• Reconstitution and Storage: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freezing/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.
• Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser261 of rat aquaporin 2.
• Purification: The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
• Concentration: 0.52 mg/ml
• Specificity: Specific for ~29k AQP2 protein phosphorylated at Ser261 in Western blots of rat kidney lysates.
• Application Info: WB: 1:1000
  DB: 1:1000
  IHC: 1:100
  Optimal working dilutions should be determined by the end user.
• Reference: Hoffert JD, Nielsen J, Yu MJ, Pisitikun T, Schleicher SM, Nielsen S, Knepper MA (2007) Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292: F691-F700.
  Hoffert JD, Fenton RA, Moeller HB, Simons B, Tchapyjnikov D, McDill BW, Yu MJ, Pisitkun T, Chen F, Knepper MA. (2008) Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27

Target Info

• Gene Symbol: AQP2
• Alias Symbols: AQP-2, aquaporin-2
• NCBI Gene Id: 25386
• Protein Name: Aquaporin-2
• Description of Target: Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking (Hoffert et al., 2007).
• Uniprot ID: P34080
• Protein Accession #: NP_037041.2
• Molecular Weight: ~29 kDa