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Catalog No: OKEH03152
Size:96T
Price: $525.00
SKU
OKEH03152
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH03152
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology AQP2 ELISA Kit (Rat) (OKEH03152) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Aqp2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Aqp2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Aqp2 captured in well.
ELISA Kit Range0.312-20ng/ml
ELISA Kit Component
ComponentAmount
Anti-Aqp2 Microplate96 Wells (12 x 8 Well strips)
Aqp2 Lyophilized Standard2
100X Biotinylated Aqp2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.16 ng/mL
SpecificityNatural and recombinant Rat Aquaporin-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolAqp2
Gene Full Nameaquaporin 2
Alias SymbolsADH water channel;AQP-2;AQP-CD;aquaporin 2 (collecting duct);aquaporin-2;aquaporin-CD;collecting duct water channel protein;water channel protein for renal collecting duct;WCH-CD.
NCBI Gene Id25386
Protein NameP34080
Description of TargetAquaporin-2
Uniprot IDhttps://www.uniprot.org/uniprot/P34080
Protein Accession #NP_037041.2
Nucleotide Accession #NM_012909.2
Protein Size (# AA)271
Molecular Weight29 kDa
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