AQP2 ELISA Kit (Rat) (OKCA00947)

Catalog No: OKCA00947

Size:96 Wells

Rat AQP2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	86	82-93
  1:2	90	85-95
  1:4	93	88-97
  1:8	94	91-98

• ELISA Kit Principle: Aviva Systems Biology AQP2 ELISA Kit (Rat) (OKCA00947) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for AQP2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AQP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample AQP2 captured in well.
• ELISA Kit Range: 6.25 - 400 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	101	95-106
  EDTA Plasma (n=4)	94	87-99

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level AQP2 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level AQP2 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • AQP2 Microplate: 96 Wells (12 x 8 Well strips)
  • AQP2 Lyophilized Standard: 2
  • 100X Biotinylated AQP2 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue Homogenates
• Sensitivity: 1.56 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: AQP2
• Gene Full Name: Aquaporin-2
• Alias Symbols: Aquaporin-2; ADH water channel; Aquaporin-CD; Collecting duct water channel protein
• Protein Name: Aquaporin-2
• Description of Target: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Plays an essential role in renal water homeostasis.
• Uniprot ID: P79213
• Protein Accession #: XP_002711176.1
• Nucleotide Accession #: XM_002711130.3