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Catalog No: OKEH06305
Size:96T
Price: $500.00
SKU
OKEH06305
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Anxa3 ELISA Kit (Rat) (OKEH06305) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Anxa3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Anxa3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Anxa3 captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 104%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <4.3%, n = 20
Mean Inter-Assay Precision - <8.7%, n = 20
ELISA Kit Component
ComponentAmount
ANXA3 Microplate96 Wells (12 x 8 Well strips)
ANXA3 Lyophilized Standard2
100X Biotinylated ANXA3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Rat Annexin A3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolAnxa3
Gene Full Nameannexin A3
Alias Symbols35-alpha calcimedin;annexin A3;Annexin III;Annexin III (Lipocortin III);annexin-3;Anx3;LC3;lipocortin III;LRRGT00047;PAP-III;placental anticoagulant protein III.
NCBI Gene Id25291
Protein NameP14669
Description of TargetAnnexin A3
Uniprot IDhttps://www.uniprot.org/uniprot/P14669
Protein Accession #NP_036955.2
Nucleotide Accession #NM_012823.2
Protein Size (# AA)324
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