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Catalog No: OKEH00384
Size:96T
Price: $525.00
SKU
OKEH00384
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ANXA3 ELISA Kit (Human) : 96 Wells (OKEH00384)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ANXA3 ELISA Kit (Human) (OKEH00384) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ANXA3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for ANXA3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ANXA3 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 7.2% (n = 20)
Mean Inter-assay CV%: < 10.7% (n = 20)
ELISA Kit Component
ComponentAmount
ANXA3 Microplate96 Wells (12 x 8 Well strips)
ANXA3 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated ANXA3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human Annexin A3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolANXA3
Gene Full Nameannexin A3
Alias Symbols35-alpha calcimedin;annexin A3;Annexin III;Annexin III (lipocortin III);annexin III (lipocortin III, 1,2-cyclic-inositol-phosphate phosphodiesterase, placental anticoagulant protein III, calcimedin 35-alpha);annexin-3;ANX3;calcimedin 35-alpha;inositol 1,2-cyclic phosphate 2-phosphohydrolase;lipocortin III;PAP-III;placental anticoagulant protein III.
NCBI Gene Id306
Protein NameP12429
Description of TargetAnnexin A3
Uniprot IDhttps://www.uniprot.org/uniprot/P12429
Protein Accession #NP_005130.1
Nucleotide Accession #NM_005139.2
Protein Size (# AA)323
Molecular Weight36 kDa
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