TGFBR1 Antibody (Phospho-S165) (OAAI00768)

Catalog No: OAAI00768

Size:100UG

WB: The cell lysate derived from TGF beta peptide (10 ng/ml for 30 minutes) stimulated MCF-7 cell lysate was immunoprobed by Rabbit anti-TGFBR1 (Cat#OAAI00768) at 1:500. An immunoreactive band is observed around ~55 kDa

IHC: Formalin-fixed, paraffin- embedded human breast carcinoma tissue stained with Rabbit anti-TGFBR1)(pSer165) antibody (Cat. #OAAI00768) at 1:50 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
  Positive Control: MCF7
  Cellular Location: Membrane/Cytoplasm
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2-8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide derived from internal sequence (-EEDP-*S-LDRP) with a phosphorylation site at Serine 165 of mouse TGFbeta receptor type-1. This sequence is also identical within human, rat, mouse, chicken, bovine and dog species.
• Purification: Affinity Purified
• Concentration: 0.5 mg/ml
• Target Post-Translational Modification: Phosphorylated
• Specificity: This antibody recognizes human protein. The other species are not tested.
• Application Info: WB: 0.1 - 1 ug/mL
  ELISA: 0.01 - 0.1 ug/mL
  IP: 2 - 5 ug/mL
  IHC: 2 - 10 ug/mL
• Reference: Wrana,J.L., et al. TGF beta signals throμgh a heteromeric protein kinase receptor Complex. Cell 71 (6), 1003-1014 (1992).
  Tomoda,T., Kudoh,T., Noma,T., Nakazawa,A., Muramatsu,M. and Arai,K.
  Molecular cloning of a Mouse counterpart for Human TGF-beta type I receptor. Biochem. Biophys. Res. Commun. 198 (3), 1054-1062 (1994)
• Publications:

  A pulsatile release platform based on photo-induced imine-crosslinking hydrogel promotes scarless wound healing. Nat Commun. 12, 1670 (2021) 33723267

  Pericyte MyD88 and IRAK4 control inflammatory and fibrotic responses to tissue injury. J Clin Invest. 127, 321-334 (2017). 27869651

  Phenotypic characterization of adenomyosis occurring at the inner and outer myometrium. PLoS One. 12, e0189522 (2017). 29253010

• Description:
  View product citations for TGFBR1 Antibody (Phospho-S165) (OAAI00768) on CiteAb

Target Info

• Gene Symbol: TGFBR1
• Gene Full Name: transforming growth factor beta receptor 1
• Alias Symbols: AAT5;activin A receptor type II-like kinase, 53kDa;activin A receptor type II-like protein kinase of 53kD;activin receptor-like kinase 5;ACVRLK4;ALK5;ALK-5;ESS1;LDS1;LDS1A;LDS2A;MSSE;mutant transforming growth factor beta receptor I;serine/threonine-protein kinase receptor R4;SKR4;tbetaR-I;TBR-i;TBRI;TGF-beta receptor type-1;TGFR-1;transforming growth factor beta receptor I;transforming growth factor-beta receptor type I.
• NCBI Gene Id: 7046
• Description of Target: Transforming growth factor beta isoforms (TGF-beta1, 2, 3) play vital roles in regulating cellular growth and differentiation, and they signal throμgh a highly restricted subset of receptors known as TGF-beta type I receptor (TbetaR-I) and TGF-beta type II receptor (TbetaR-II). Expression of T beta R-I is more ubiquitous than that of T beta R-II. There was no staining of T beta R-II in the central nervous system, while intense T beta R-I staining was found specifically in nervous tissues. Expression of T beta R-I and T beta R-II was mostly coincident with that of their ligands, sμggesting that TGF-beta s act as multiple mediators during organogenesis. TGF-beta binds with high affinity to its receptor, a transmembrane protein with a cytoplasmic serine/threonine kinase domain, to form a dynamic dimer with phosphorylation in the cytoplasmic Ser/Thr residuals which cause its kinase activity and association with another TGF beta-binding protein, the type I receptor, to signal growth inhibition and early gene responses.
  
• Uniprot ID: P36897
• Molecular Weight: 55