TGFBR1 Antibody (OAAF01161)

Catalog No: OAAF01161 (Formerly GWB-ASC556)

Size:100 ug

Western blot analysis of extracts from HepG2/COLO cells, using TGF beta Receptor I Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using TGF beta Receptor I Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human TGF beta Receptor I.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Specificity: TGF beta Receptor I Antibody detects endogenous levels of total TGF beta Receptor I protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:1000; IF: 1:100~1:500; ELISA: 1:10000
• Reference: Franzen P., Cell 75:681-692(1993).
  Vellucci V.F., Genomics 46:278-283(1997).
  Lynch M.A., Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.

Target Info

• Gene Symbol: TGFBR1
• Gene Full Name: transforming growth factor beta receptor 1
• Alias Symbols: AAT5;activin A receptor type II-like kinase, 53kDa;activin A receptor type II-like protein kinase of 53kD;activin receptor-like kinase 5;ACVRLK4;ALK5;ALK-5;ESS1;LDS1;LDS1A;LDS2A;MSSE;mutant transforming growth factor beta receptor I;serine/threonine-protein kinase receptor R4;SKR4;tbetaR-I;TBR-i;TBRI;TGF-beta receptor type-1;TGF-beta type I receptor;TGFR-1;transforming growth factor beta receptor I;transforming growth factor-beta receptor type I.
• NCBI Gene Id: 7046
• Protein Name: TGF-beta receptor type-1
• Description of Target: Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation.
• Uniprot ID: P36897
• Protein Accession #: NP_001124388.1
• Molecular Weight: 55 kDa