Catalog No: OKEH01857
Size:96T
Price: $675.00
SKU
OKEH01857
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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TGFBR1 ELISA Kit (Human) : 96 Wells (OKEH01857)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TGFBR1 ELISA Kit (Human) (OKEH01857) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TGFBR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TGFBR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TGFBR1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 9.0% (n = 20)
ELISA Kit Component
ComponentAmount
TGFBR1 Microplate96 Wells (12 x 8 Well strips)
TGFBR1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated ACTB Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human TGF-beta receptor type-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTGFBR1
Gene Full Nametransforming growth factor beta receptor 1
Alias SymbolsAAT5;activin A receptor type II-like kinase, 53kDa;activin A receptor type II-like protein kinase of 53kD;activin receptor-like kinase 5;ACVRLK4;ALK5;ALK-5;ESS1;LDS1;LDS1A;LDS2A;MSSE;mutant transforming growth factor beta receptor I;serine/threonine-protein kinase receptor R4;SKR4;tbetaR-I;TBR-i;TBRI;TGF-beta receptor type-1;TGF-beta type I receptor;TGFR-1;transforming growth factor beta receptor I;transforming growth factor-beta receptor type I.
NCBI Gene Id7046
Protein NameP36897
Description of TargetTGF-beta receptor type-1
Uniprot IDhttps://www.uniprot.org/uniprot/P36897
Protein Accession #NP_001124388.1
Nucleotide Accession #NM_001130916.2
Protein Size (# AA)503
Molecular Weight56 kDa
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