Anti-Nestin (OAPC00021)

Catalog No: OAPC00021

Size:100 ul

Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~220-240 k nestin doublet.

Immunochemical staining of cultured neonatal rat neurons and glia showing nestin labeling in red and vimentin (cat. # OAPC00084) in green. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS with 50% glycerol and 5mM sodium azide. Adequate amount of material to conduct 10-mini Western blots
• Clonality: Monoclonal
• Clone: 4D11
• Isotype: IgG1
• Host: Mouse
• Application: WB, IF
• Reconstitution and Storage: Store at -20°C; stable for at least one year. Avoid freeze/thaw cycles.
• Immunogen: Recombinant partial segment of human nestin expressed in and purified from E.coli.
• Purification: Protein G purified
• Concentration: Varies by lot. See vial for concentration.
• Specificity: Specific for the ~220-240 kDa nestin doublet in Western blot in neonatal rat brain homogenate.
• Application Info: WB: 1:2,000
  IF: 1:500
  Optimal dilutions should be determined by the end user.

Target Info

• Gene Symbol: NES
• Alias Symbols: Nbla00170
• NCBI Gene Id: 10763
• Protein Name: Nestin
• Description of Target: Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells (Lendahl et al., 1990). Nestin was originally identified as a result of the production of a series of monoclonal antibodies directed against epitopes expressed on formalin fixed embryo day 15 rat spinal cord (Hockfield et al., 1985)). One of these antibodies, called Rat 401, stained fibrous profiles in the developing nervous system, but not in the mature nervous system. By screening bacteriophage expression libraries with Rat 401, Lendahl et al. were able to isolate a cDNA encoding the protein to which Rat 401 antibody bound. The protein proved to be an unusual member of the intermediate filament family, containing an alpha-helical region homologous to that found in keratin and neurofilament subunits.
• Uniprot ID: P48681
• Protein Accession #: NP_006608
• Nucleotide Accession #: NM_006617
• Molecular Weight: ~220-240kDa