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Anti-Actin (OAPC00015)

100 ul
$289.00
In Stock
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Gene Symbol:
ACTA1
NCBI Gene Id:
421534
Protein Name:
Actin, alpha skeletal muscle
Swissprot Id:
P68139
Protein Accession #:
NP_001026234.1
Replacement Item:
This antibody may replace item sc-10731 from Santa Cruz Biotechnology.
Description of Target:
Actin is the most abundant protein found in virtually all eukaryotic cells. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans, making it an excellent choice for use as a loading control. It is the monomeric subunit of microfilaments, one of the three major components of the cytoskeleton, and of thin filaments, which are part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular functions, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape.
Molecular Weight:
~42kDa
Host:
Mouse
Clonality:
Monoclonal
Purification:
Unpurified ascites fluid.
Application:
WB, IF, IHC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ACTA1.
RNA Seq:
Find tissues and cell lines supported by DNA array analysis to express ACTA1.
Immunogen:
Chicken gizzard actin.
Predicted Species Reactivity:
Zebrafish
Predicted Homology Based on Immunogen Sequence:
Zebrafish
Product Format:
Liquid containing 0.01% sodium azide.
Reconstitution and Storage:
Store at -20C in undiluted aliquots; stable for at least one year. Avoid freeze/thaw cycles.
Datasheets/Manuals:
Printable datasheet for OAPC00015
Specificity:
Specific for the ~42 kDa actin protein in lysates from skeletal, cardiac, gizzard and aorta tissues. Reacts with all actin isoforms.
Clone:
C4
Isotype:
IgG1
Application Info:
The antibody reacts with all known actins from yeast to man.
Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 42k actin protein.
Additional Information:
Packaging :
Liquid containing 0.01% sodium azide.
Target Reference:
Lessard, JL. Two monoclonal antibodies to actin: one muscle selective and one generally reactive. Cell Motility and Cytoskeleton (1998) 10:349-362.

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