Product Number |
OAPC00015 |
Product Page |
www.avivasysbio.com/anti-actin-oapc00015.html |
Name |
Anti-Actin (OAPC00015) |
Molecular Weight |
~42kDa |
Clone |
C4 |
Isotype |
IgG1 |
NCBI Gene Id |
421534 |
Host |
Mouse |
Clonality |
Monoclonal |
Product Format |
Liquid containing 0.01% sodium azide. |
Reference |
Lessard, JL. Two monoclonal antibodies to actin: one muscle selective and one generally reactive. Cell Motility and Cytoskeleton (1998) 10:349-362. |
Description of Target |
Actin is the most abundant protein found in virtually all eukaryotic cells. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans, making it an excellent choice for use as a loading control. It is the monomeric subunit of microfilaments, one of the three major components of the cytoskeleton, and of thin filaments, which are part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular functions, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. |
Reconstitution and Storage |
Store at -20C in undiluted aliquots; stable for at least one year. Avoid freeze/thaw cycles. |
Datasheets/Manuals |
Printable datasheet for OAPC00015 |
Specificity |
Specific for the ~42 kDa actin protein in lysates from skeletal, cardiac, gizzard and aorta tissues. Reacts with all actin isoforms. |
Additional Information |
Packaging : Liquid containing 0.01% sodium azide. |
Application Info |
The antibody reacts with all known actins from yeast to man. Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 42k actin protein.
|
Immunogen |
Chicken gizzard actin. |
Uniprot ID |
P68139 |
Protein Name |
Actin, alpha skeletal muscle |
Protein Accession # |
NP_001026234.1 |
Purification |
Unpurified ascites fluid. |
Gene Symbol |
ACTA1 |
Predicted Species Reactivity |
Zebrafish |
Application |
WB, IF, IHC |
Predicted Homology Based on Immunogen Sequence |
Zebrafish |
Image 1 | Rat hippocampal homogenate
| Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 42k actin protein. |
|
|