ACTA1 Antibody (OAAB06896)

Catalog No: OAAB06896

Size:400ul

ACTA1 Antibody(Cat. #OAAB06896) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ACTA1 antibody detected the ACTA1 protein (arrow).

ACTA1 Antibody (Cat. #OAAB06896)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

ACTA1 Antibody (Cat. #OAAB06896)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

ACTA1 Antibody (Cat. #OAAB06896) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Monoclonal
• Clone: 337CT30.10.1
• Isotype: IgG1,k
• Host: Mouse
• Application: WB, IHC-P, FC
  Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  Cellular Location: Cytoplasm, cytoskeleton.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ACTA1 monoclonal antibody is generated from mouse immunized with ACTA1 recombinant protein.
• Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Bovine, Chicken, Mouse, Pig, Rabbit, Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  WB: 1:500~1:1000
  IHC-P: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: ACTA1
• Alias Symbols: ACTA, ASMA, CFTD, MPFD, NEM1, NEM2, NEM3, SHPM, CFTD1, CFTDM
• NCBI Gene Id: 58
• Protein Name: Actin, alpha skeletal muscle
• Description of Target: The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
• Uniprot ID: P68133
• Protein Accession #: NP_001091.1
• Molecular Weight: 42 kDa