Catalog No: OKEH02626
Price: $525.00
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ACE2 ELISA Kit (Human) : 96 Wells (OKEH02626)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02626
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ACE2 ELISA Kit (Human) (OKEH02626) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACE2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ACE2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACE2 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 8.7% (n = 20)
ELISA Kit Component
Anti-ACE2 Microplate96 Wells (12 x 8 Well strips)
ACE2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated ACE2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.34 ng/mL
SpecificityNatural and recombinant Human Angiotensin-converting enzyme 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolACE2
Gene Full Nameangiotensin converting enzyme 2
Alias SymbolsACEH;ACE-related carboxypeptidase;angiotensin I converting enzyme (peptidyl-dipeptidase A) 2;angiotensin I converting enzyme 2;angiotensin-converting enzyme 2;angiotensin-converting enzyme homolog;angiotensin-converting enzyme-related carboxypeptidase;metalloprotease MPROT15;peptidyl-dipeptidase A;truncated angiotensin converting enzyme 2.
NCBI Gene Id59272
Protein NameQ9BYF1
Description of TargetAngiotensin-converting enzyme 2
Uniprot IDhttps://www.uniprot.org/uniprot/Q9BYF1
Protein Accession #NP_068576.1
Nucleotide Accession #NM_021804.2
Protein Size (# AA)805
Molecular Weight92 kDa
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