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Datasheets/Manuals | Printable datasheet for OAPB00213 |
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Predicted Species Reactivity | Human, Mouse, Rat |
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Product Format | Liquid. Supplied in PBS containing 0.02% sodium azide. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Conjugation | Unconjugated |
Application | ELISA, WB, IHC-P, IF |
Additional Information | Positive Control: Human Kidney Tissue Lysate, Human Kidney Tissue Slide, Human Spleen Tissue Lysate, Human Testis Tissue Lysate |
:: | Isoforms: Human ACE2 has 2 isoforms, including isoform 1 (805aa, 93kDa) and isoform 2 (555aa, 64kDa). Mouse ACE2 also has 2 isoforms, including isoform 1 (805aa, 92kDa) and isoform 2 (353aa, 40kDa). Rat ACE2 has one isoform (805aa, 93kDa). This product can detect human, mouse and rat. |
Reconstitution and Storage | Stored at 4C for three months and -20C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Immunogen | Anti-ACE2 antibody was raised against a peptide corresponding to 17 amino acids near the center of human ACE2. The immunogen is located within amino acids 180 - 230 of ACE2. |
Purification | ACE2 Antibody is affinity chromatography purified via peptide column. |
Predicted Homology Based on Immunogen Sequence | Predicted species reactivity based on immunogen sequence: Bovine: (94%) |
Concentration | 1 mg/mL |
Blocking Peptide | For anti-ACE2 antibody is Catalog # OEPB02307 |
Specificity | Anti-ACE2 has no cross response to ACE1. |
Application Info | WB: 1-2 ug/mL. IHC: 2 ug/mL. IF: 20 ug/mL. Antibody validated: Western Blot in human, mouse and rat samples. Immunohistochemistry in human samples. Immunofluorescence in human samples. All other applications and species not yet tested. |
Reference | Donoghue et al. Circ. Res. 2000;87:1-9. Tipnis et al. J Biol. Chem. 2000;275:33238-43. Li et al. Nature 2003;426:450-4. Lu et al. The Lancet 2020 (published online). Crackower et al. Nature 2002;417:822-8. |
Gene Symbol | ACE2 |
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Gene Full Name | angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 |
Alias Symbols | ACEH |
NCBI Gene Id | 59272 |
Protein Name | Angiotensin-converting enzyme 2 |
Description of Target | Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. Homology modeling shows 2019-nCoV has a similar receptor-binding domain structure as SARS-CoV, which suggests COVID-19 (2019-nCoV) may use ACE2 as a receptor in humans for infection. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function. |
Uniprot ID | Q9BYF1 |
Protein Accession # | NP_068576 |
Molecular Weight | Predicted: 93kDa Observed: 130 kDa (7 N-linked glycosylation) |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ACE2 Antibody (OAPB00213)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "ACE2 Antibody (OAPB00213)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "ACE2 Antibody (OAPB00213)" provided in?
This item is provided in "Liquid. Supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ACE2 Antibody (OAPB00213)"?
This target may also be called "ACEH" in publications.
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What is the shipping cost for "ACE2 Antibody (OAPB00213)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ACE2 Antibody (OAPB00213)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ACE2 Antibody (OAPB00213)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "Predicted: 93kDa
Observed: 130 kDa (7 N-linked glycosylation) ".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ACE2 Antibody (OAPB00213)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ACE2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ACE2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ACE2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ACE2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ACE2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ACE2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.