ACE2 Antibody (OAPB00213)

Catalog No: OAPB00213 (Formerly GWB-D7D98E)

Size:0.1 mg

Figure 2 Western Blot Validation in Human Tissues
Loading: 15 ug of lysates per lane. Antibodies: ACE2 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 3 Western Blot Validation in Mouse Tissues
Loading: 15 ug of lysates per lane. Antibodies: ACE2 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 4 Western Blot Validation in Rat Skin Tissue
Loading: 15 ug of lysates per lane. Antibodies: ACE2 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Tissues (kidney, spleen testis, breast, intestine)
Loading: 15 ug of lysates per lane. Antibodies: OAPB00213 (2 ug/mL), OAPB00214 (2 ug/mL), OAPB00219 (2 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 5 Immunohistochemistry Validation of ACE2 in Human Kidney Tissue
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 6 Immunofluorescence Validation of ACE2 in Human Kidney Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney cells labeling ACE2 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: ELISA, WB, IHC-P, IF
• Additional Information: Positive Control: Human Kidney Tissue Lysate, Human Kidney Tissue Slide, Human Spleen Tissue Lysate, Human Testis Tissue Lysate
  Isoforms: Human ACE2 has 2 isoforms, including isoform 1 (805aa, 93kDa) and isoform 2 (555aa, 64kDa). Mouse ACE2 also has 2 isoforms, including isoform 1 (805aa, 92kDa) and isoform 2 (353aa, 40kDa). Rat ACE2 has one isoform (805aa, 93kDa). This product can detect human, mouse and rat.
• Reconstitution and Storage: Stored at 4C for three months and -20C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Anti-ACE2 antibody was raised against a peptide corresponding to 17 amino acids near the center of human ACE2.
  The immunogen is located within amino acids 180 - 230 of ACE2.
• Purification: ACE2 Antibody is affinity chromatography purified via peptide column.
• Predicted Homology Based on Immunogen Sequence: Predicted species reactivity based on immunogen sequence: Bovine: (94%)
• Concentration: 1 mg/mL
• Blocking Peptide: For anti-ACE2 antibody is Catalog # OEPB02307
• Specificity: Anti-ACE2 has no cross response to ACE1.
• Application Info: WB: 1-2 ug/mL. IHC: 2 ug/mL. IF: 20 ug/mL.
  Antibody validated: Western Blot in human, mouse and rat samples. Immunohistochemistry in human samples. Immunofluorescence in human samples. All other applications and species not yet tested.
• Reference: Donoghue et al. Circ. Res. 2000;87:1-9.
  Tipnis et al. J Biol. Chem. 2000;275:33238-43.
  Li et al. Nature 2003;426:450-4.
  Lu et al. The Lancet 2020 (published online).
  Crackower et al. Nature 2002;417:822-8.

Target Info

• Gene Symbol: ACE2
• Gene Full Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• Alias Symbols: ACEH
• NCBI Gene Id: 59272
• Protein Name: Angiotensin-converting enzyme 2
• Description of Target: Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. Homology modeling shows 2019-nCoV has a similar receptor-binding domain structure as SARS-CoV, which suggests COVID-19 (2019-nCoV) may use ACE2 as a receptor in humans for infection. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.
• Uniprot ID: Q9BYF1
• Protein Accession #: NP_068576
• Molecular Weight: Predicted: 93kDa
  Observed: 130 kDa (7 N-linked glycosylation)