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Datasheets/Manuals | Printable datasheet for ACE2 Antibody (OAPB00213) (OAPB00213) |
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Predicted Species Reactivity | Bovine|Human|Mouse|Rat |
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Product Format | Liquid. ACE2 Antibody is supplied in PBS containing 0.02% sodium azide. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot |
Additional Information | Positive Control: Human Kidney Tissue Lysate, Human Kidney Tissue Slide, Human Spleen Tissue Lysate, Human Testis Tissue Lysate |
:: | Isoforms: Human ACE2 has 2 isoforms, including isoform 1 (805aa, 93kDa) and isoform 2 (555aa, 64kDa). Mouse ACE2 also has 2 isoforms, including isoform 1 (805aa, 92kDa) and isoform 2 (353aa, 40kDa). Rat ACE2 has one isoform (805aa, 93kDa). This product can detect human, mouse and rat. |
Reconstitution and Storage | Stored at 4C for three months and -20C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Immunogen | Anti-ACE2 antibody (3215) was raised against a peptide corresponding to 17 amino acids near the center of human ACE2. The immunogen is located within amino acids 180 - 230 of ACE2. |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Predicted species reactivity based on immunogen sequence: Bovine: (94%) |
Concentration | 1 mg/ml |
Blocking Peptide | For anti-ACE2 antibody is Catalog # OEPB02307 |
Specificity | Anti-ACE2 has no cross response to ACE1. |
Application Info | WB: 1-2 ug/mL. IHC: 2 ug/mL. IF: 20 ug/mL. Antibody validated: Western Blot in human, mouse and rat samples. Immunohistochemistry in human samples. Immunofluorescence in human samples. All other applications and species not yet tested. |
Reference | Donoghue et al. Circ. Res. 2000;87:1-9. Tipnis et al. J Biol. Chem. 2000;275:33238-43. Li et al. Nature 2003;426:450-4. Lu et al. The Lancet 2020 (published online). Crackower et al. Nature 2002;417:822-8. |
Gene Symbol | ACE2 |
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Gene Full Name | angiotensin converting enzyme 2 |
Alias Symbols | ACEH;ACE-related carboxypeptidase;angiotensin I converting enzyme (peptidyl-dipeptidase A) 2;angiotensin I converting enzyme 2;angiotensin-converting enzyme 2;angiotensin-converting enzyme homolog;angiotensin-converting enzyme-related carboxypeptidase;metalloprotease MPROT15;peptidyl-dipeptidase A;truncated angiotensin converting enzyme 2. |
NCBI Gene Id | 59272 |
Protein Name | Angiotensin-converting enzyme 2|angiotensin-converting enzyme 2 precursor [Homo sapiens] |
Description of Target | Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed:10969042, PubMed:10924499, PubMed:11815627, PubMed:19021774, PubMed:14504186). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed:10969042, PubMed:11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed:11815627, PubMed:27217402, PubMed:28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed:18424768, PubMed:19185582). |
Uniprot ID | Q9BYF1 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_068576 |
Molecular Weight | Predicted: 93kD Observed: 130 kD (7 N-linked glycosylation) |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ACE2 Antibody (OAPB00213)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Human|Mouse|Rat".
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How long will it take to receive "ACE2 Antibody (OAPB00213)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "ACE2 Antibody (OAPB00213)" provided in?
This item is provided in "Liquid. ACE2 Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ACE2 Antibody (OAPB00213)"?
This target may also be called "ACEH;ACE-related carboxypeptidase;angiotensin I converting enzyme (peptidyl-dipeptidase A) 2;angiotensin I converting enzyme 2;angiotensin-converting enzyme 2;angiotensin-converting enzyme homolog;angiotensin-converting enzyme-related carboxypeptidase;metalloprotease MPROT15;peptidyl-dipeptidase A;truncated angiotensin converting enzyme 2." in publications.
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What is the shipping cost for "ACE2 Antibody (OAPB00213)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ACE2 Antibody (OAPB00213)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ACE2 Antibody (OAPB00213)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "Predicted: 93kD Observed: 130 kD (7 N-linked glycosylation)".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ACE2 Antibody (OAPB00213)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ACE2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ACE2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ACE2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ACE2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ACE2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ACE2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.