WNT3 Chemi-Luminescent ELISA Kit (Human) (OKCD04041)

Catalog No: OKCD04041

Size:96WELLS

Human WNT3 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	85-94%	78-99%	87-99%	78-96%
  EDTA Plasma (n=5)	82-95%	96-104%	80-96%	92-101%
  Heparin Plasma (n=5)	78-95%	79-105%	84-93%	86-99%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 3 (WNT3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 3 (WNT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wingless Type MMTV Integration Site Family, Member 3 (WNT3) level in the sample or standard.
• ELISA Kit Range: 41.2-30,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	80-91	86
  EDTA Plasma (n=5)	98-105	101
  Heparin Plasma (n=5)	85-92	89

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 3 (WNT3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 3 (WNT3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • WNT3 Microplate: 96 Wells (12 x 8 Well strips)
  • WNT3 Lyophilized Standard: 2
  • 100X WNT3 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.0412 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: WNT3
• Gene Full Name: Wnt family member 3
• Alias Symbols: INT4;proto-oncogene Int-4 homolog;proto-oncogene Wnt-3;TETAMS;wingless-type MMTV integration site family, member 3;WNT-3 proto-oncogene protein.
• NCBI Gene Id: 7473
• Protein Name: Proto-oncogene Wnt-3
• Description of Target: Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:26902720). Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis. Required for normal formation of the apical ectodermal ridge (By similarity). Required for normal embryonic development, and especially for limb development (PubMed:14872406).
• Uniprot ID: P56703
• Protein Accession #: NP_110380
• Nucleotide Accession #: NM_030753