WNT3 ELISA Kit (Human) (OKCD00611)

Catalog No: OKCD00611 (Formerly GWB-KBBWD9)

Size:96WELLS

Human WNT3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	92-102%	84-97%	84-99%	91-98%
  EDTA Plasma (n=5)	79-94%	78-102%	79-97%	96-104%
  Heparin Plasma (n=5)	82-103%	80-98%	85-92%	85-96%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 3 (WNT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 3 (WNT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 3 (WNT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 3 (WNT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	80-89	84
  EDTA plasma(n=5)	80-96	86
  heparin plasma(n=5)	81-91	85

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 3 (WNT3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 3 (WNT3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-WNT3 Microplate: 96 Wells (12 x 8 Well Strips)
  • WNT3 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated WNT3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.054 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 3 (WNT3). No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 3 (WNT3) and analogues was observed.
• Publications:

  Nicotine enhances mesangial cell proliferation and fibronectin production in high glucose milieu via activation of Wnt/β-catenin pathway. Biosci Rep. 38, (2018). 29572389

• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Description:
  View product citations for WNT3 ELISA kit (Human) on CiteAb

Target Info

• Gene Symbol: WNT3
• Gene Full Name: Wnt family member 3
• Alias Symbols: INT4;proto-oncogene Int-4 homolog;proto-oncogene Wnt-3;TETAMS;wingless-type MMTV integration site family, member 3;WNT-3 proto-oncogene protein.
• NCBI Gene Id: 7473
• Protein Name: Proto-oncogene Wnt-3
• Description of Target: Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:26902720). Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis. Required for normal formation of the apical ectodermal ridge (By similarity). Required for normal embryonic development, and especially for limb development (PubMed:14872406).
• Uniprot ID: P56703
• Protein Accession #: NP_110380
• Nucleotide Accession #: NM_030753.4
• Protein Size (# AA): 355