VIM ELISA Kit (Rat) (OKCA02611)

Catalog No: OKCA02611

Size:96 Wells

Rat VIM ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	88	80-95
  1:2	93	88-98
  1:4	97	93-102
  1:8	100	95-104

• ELISA Kit Principle: Aviva Systems Biology Vim ELISA Kit (Rat) (OKCA02611) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Vim has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Vim is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Vim captured in the well.
• ELISA Kit Range: 23.5 - 1,500 ng/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	102	95-107
  EDTA Plasma (n=4)	90	85-94

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • Vim Microplate: 96 Wells (12 x 8 Well strips)
  • Vim Lyophilized Standard: 2
  • 100X Biotinylated Vim Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 5.8 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: VIM
• Alias Symbols: RP11-124N14.1, FLJ36605
• NCBI Gene Id: 81818
• Protein Name: Vimentin
• Description of Target: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
• Uniprot ID: P31000
• Protein Accession #: NP_112402
• Nucleotide Accession #: NM_031140