VIMENTIN Antibody (OAAD00212)

Catalog No: OAAD00212 (Formerly GWB-BBB094)

Size:100 ug

Western blot At 0.5-1?g/ml with the appropriate system to detect vimentin in cells and tissues.

Product Info

• Predicted Species Reactivity: Human, Rat
• Clonality: Monoclonal
• Clone: VMT-24
• Isotype: IgG1
• Host: Mouse
• Application: WB, IHC, IP
• Reconstitution and Storage: At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time.
• Immunogen: Pig eye lens vimentin.
• Purification: Purified by the goat anti-mouse IgG Affinity chromatography.
• Specificity: Human, rat.
  No cross reactivity with other proteins.
• Formulation: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative.
• Application Info: Western blot At 0.5-1ug/ml with the appropriate system to detect vimentin in cells and tissues.
  Immunohistochemistry(P) At 1.5-2.5ug/ml to detect vimentin in formalin fixed and paraffin embedded tissues.
  Other applications have not been tested.
  Optimal dilutions should be determined by end user.
• Storage Buffer: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
• Reference: 1. Lilienbaum, A.; Legagneux, V.; Portier, M.-M.; Dellagi, K.; Paulin, D. : Vimentin gene: expression in human lymphocytes and in Burkitt's lymphoma cells. EMBO J. 5: 2809-2814, 1986.
  2. Mor-Vaknin, N.; Punturieri, A.; Sitwala, K.; Markovitz, D. M. : Vimentin is secreted by activated macrophages. Nature Cell Biol. 5: 59-63, 2003 3 Ferrari, S.; Cannizzaro, L. A.; Battini, R.; Huebner, K.; Baserga, R. : The gene encoding human vimentin is located on the short arm of chromosome 10. Am. J. Hum. Genet. 41: 616-626, 1987.

Target Info

• Gene Symbol: VIM
• Alias Symbols: HEL113, CTRCT30,
• NCBI Gene Id: 7431
• Protein Name: Vimentin
• Description of Target: The VIM gene was one of many that Gieser and Swaroop (1992) recovered from a subtracted cDNA library for retinal pigment epithelium. Vimentin gene express in human lymphocytes and in Burkitt's lymphoma cells. Vimentin is secreted by activated macrophages. The gene encoding human vimentin is located on the short arm of chromosome 10.
• Uniprot ID: P08670