VIL1 Antibody (OAGA03505)

Catalog No: OAGA03505

Size:100 ul

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membranes were blotted with Villin antibody diluted at 1:1000 and competitor's antibody (sc-7672) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Villin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
Diluted at 1:10000

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Villin antibody at 1:500 dilution.

Immunofluorescence analysis of methanol-fixed Hep3B, using Villin antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Villin. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: VIL1
• Gene Full Name: villin 1
• Alias Symbols: VIL, D2S1471
• NCBI Gene Id: 7429
• Protein Name: villin-1
• Description of Target: This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon.
• Uniprot ID: P09327
• Protein Accession #: NP_009058.2
• Nucleotide Accession #: NM_007127.2