UHRF1 Antibody - middle region (OAAB05441)

Catalog No: OAAB05441

Size:400UL

UHRF1 Antibody (Center) (Cat. #OAAB05441) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UHRF1 antibody detected the UHRF1 protein (arrow).

UHRF1 Antibody (Center) (Cat.#OAAB05441) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: FC,IF,WB
  Description: This UHRF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236~265 amino acids from the Center region of human UHRF1.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This UHRF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-257 amino acids from the Central region of human UHRF1.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  FC: 1:10~50
  IF: 1:10~50
• Other Applications Image 1 Data: UHRF1 Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: UHRF1
• Alias Symbols: E3 ubiquitin-protein ligase UHRF1, hNP95, hUHRF1, huNp95, ICBP90, inverted CCAAT box-binding protein of 90 kDa, Np95, nuclear phosphoprotein 95, nuclear protein 95, nuclear zinc finger protein Np95, RING finger protein 106, RING-type E3 ubiquitin transferase UHRF1, RNF106, TDRD22, transcription factor ICBP90, ubiquitin-like PHD and RING finger domain-containing protein 1, Ubiquitin-like-containing PHD and RING finger domains protein 1.
• NCBI Gene Id: 29128
• Protein Name: E3 ubiquitin-protein ligase UHRF1|E3 ubiquitin-protein ligase UHRF1 isoform 1 [Homo sapiens]|E3 ubiquitin-protein ligase UHRF1 isoform 2 [Homo sapiens]
• Description of Target: Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair.
• Uniprot ID: Q96T88
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001041666.1,NP_037414.3
• Molecular Weight: 89814