Tnfrsf11a ELISA Kit (Mouse) (OKEH04489)

Catalog No: OKEH04489

Size:96T

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Tnfrsf11a ELISA Kit (Mouse) (OKEH04489) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Tnfrsf11a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and washed. After washing, a biotinylated detector antibody specific for Tnfrsf11a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tnfrsf11a captured in well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 5.7% (n = 20)
  Mean Inter-assay CV%: <= 7.9% (n = 20)
• ELISA Kit Component:
  • Anti-Tnfrsf11a Microplate: 96 Wells (12 x 8 Well Strips)
  • Tnfrsf11a Lyophilized Standard: 2 x 10 ng
  • Sample Diluent: 1 x 20 mL
  • 100X Tnfrsf11a Biotinylated Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 ÂuL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 15.62 pg/mL
• Specificity: Natural and recombinant Mouse Tumor necrosis factor receptor superfamily member 11A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Tnfrsf11a
• Gene Full Name: tumor necrosis factor receptor superfamily, member 11a, NFKB activator
• Alias Symbols: Ly109;mRANK;ODFR;OFE;osteoclast differentiation factor receptor;Rank;receptor activator of NF-kappaB;receptor activator of NF-KB;TRANCE-R;tumor necrosis factor receptor superfamily member 11A.
• NCBI Gene Id: 21934
• Protein Name: O35305
• Description of Target: Tumor necrosis factor receptor superfamily member 11A
• Uniprot ID: https://www.uniprot.org/uniprot/O35305
• Protein Size (# AA): 625
• Molecular Weight: 66.6 kDa