TNFRSF11A Antibody (OAPB00460)

Catalog No: OAPB00460

Size:100UG

Western blot analysis of sRANK-L in rat liver tissue lysate with sRANK-L antibody at (A) 0.25 and (B) 0.5 ug/mL.

Immunohistochemistry of sRANK-L in human liver tissue with sRANK-L antibody at 5 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. sRANK Ligand Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: sRANK Ligand antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Anti-sRANK-L antibody (3963) was raised against a peptide corresponding to 14 amino acids near the center of human sRANK-L. The immunogen is located within amino acids 140-190 of sRANK-L.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-TNFRSF11A (OAPB00460) antibody is Catalog # OEPB02570
• Application Info: sRANK-L antibody can be used for detection of sRANK-L by Western blot at 0.25 - 0.5 ug/ml. (Optimal dilution should be determined by user)
• Reference: Wong BR, Rho J, Arron J, et al. TRANCE is a novel ligand of the tumor necrosis factor receptor family that activates c-Jun N-terminal kinase in T cells. J. Biol. Chem.1997; 272:25190-4.;Kong YY, Yoshida H, Sarosi I, et al. OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis. Nature 1999; 397:315-23.;Weitzmann MN, Cenci S, Rifas L, et al. Interleukin-7 stimulates osteoclast formation by up-regulating the T-cell production of soluble osteoclastogenic cytokines. Blood 2000; 96:1873-8.;Bharti AC, Takada Y, Shishodia S, et al. Evidence that receptor activator of the nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism. J. Biol. Chem.2004; 279:6065-76.

Target Info

• Gene Symbol: TNFSF11
• Gene Full Name: TNF superfamily member 11
• Alias Symbols: CD254;hRANKL2;ODF;OPGL;OPTB2;osteoclast differentiation factor;osteoprotegerin ligand;RANKL;receptor activator of nuclear factor kappa B ligand;Receptor activator of nuclear factor kappa-B ligand;sOdf;TNF-related activation-induced cytokine;TNLG6B;TRANCE;tumor necrosis factor (ligand) superfamily, member 11;tumor necrosis factor ligand 6B;tumor necrosis factor ligand superfamily member 11;tumor necrosis factor superfamily member 11.
• NCBI Gene Id: 8600
• Protein Name: Tumor necrosis factor ligand superfamily member 11|tumor necrosis factor ligand superfamily member 11 isoform 1 [Homo sapiens]
• Description of Target: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed:22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).
• Uniprot ID: O14788
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_003692
• Nucleotide Accession #: NM_001270949.1; NM_001270950.1; NM_001270951.1; NM_001278268.1; NM_003839.3
• Molecular Weight: Predicted: 36kD Observed: 36 kD