TGF-beta 2 ELISA Kit (Mouse) (OKBB00483)

Catalog No: OKBB00483

Size:96 Wells

Mouse TGF-beta 2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse TGF-beta 2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TGF-beta 2 has been precoated onto 96-well plates. Standards(NS0, A303-S414) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF-beta 2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse TGF-beta 2 amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	242	524	1096	253	487	1295
  SD	10.9	28.3	73.43	13	35.55	90.65
  CV%	4.5	5.4	6.7	5.1	7.3	7

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse TGF-beta 2 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse TGF-beta 2 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse TGF-beta 2 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse TGF-beta 2
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A303-S414
• Description: For quantitative detection of activated mouse TGF-beta 2 in cell culture supernatants, serum and plasma(heparin, EDTA, citrate).

Target Info

• Gene Symbol: TGFB2
• Gene Full Name: transforming growth factor, beta 2
• Alias Symbols: BSC 1 cell growth inhibitor, BSC-1 cell growth inhibitor, Cetermin, G-TSF, Glioblastoma derived T cell suppressor factor, Glioblastoma-derived T-cell suppressor factor, Polyergin, TGF beta2, TGF-beta-2, TGFB2, TGFB2_HUMAN, Transforming growth factor beta-2, Transforming growth factor, beta 2
• NCBI Gene Id: 21808
• Protein Name: Transforming growth factor beta-2
• Description of Target: Transforming growth factor-beta 2 (TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma (POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured human anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.
• Uniprot ID: P27090
• Protein Accession #: NP_033393.2
• Nucleotide Accession #: NM_009367.3