Testosterone ELISA Kit (Rat) (OKCA00179)

Catalog No: OKCA00179

Size:96 Wells

Rat Testosterone ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: < 4 Hours
• ELISA Kit Linearity:

  Sample Type 1
  Sample Type	Dilution	Average %	Range %
  Serum (n=4)	1:1	91%	86-96%
  Serum (n=4)	1:2	97%	92-104%
  Serum (n=4)	1:4	85%	81-88%
  Serum (n=4)	1:8	89%	83-93%

• ELISA Kit Principle: Aviva Systems Biology Testosterone ELISA Kit (Rat) (OKCA00179) is based on a competitive enzyme immuno assay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for Testosterone and Horseradish Peroxidase (HRP) conjugated Testosterone. The competitive inhibition reaction is launched between with HRP labeled Testosterone and unlabeled Testosterone with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Testosterone in the sample. The color development is stopped and the intensity of the color is measured.
• ELISA Kit Range: 0.13 ng/mL - 25.6 ng/mL
• ELISA Kit Recovery:

  Sample 1
  Sample Type	Average Recovery	Range
  Serum (n = 5)	0.9	87-95%

  Sample 2
  Sample Type	Average Recovery	Range
  EDTA plasma (n = 4)	0.94	90-98%

• ELISA Kit Reproducibility:

  	Mean Intra-Assay Precision %CV			Mean Inter-Assay Precision %CV
  Sample	1	2	3	1	2	3
  n	20	20	20	20	20	20
  Mean (ng/mL	1.686	1.566	1.568	1.573	1.585	1.548
  SD	0.039	0.046	0.04	0.045	0.051	0.028
  CV%	6.435	7.435	6.468	7.288	8.284	4.498

• ELISA Kit Component:
  • Anti-Testosterone Microplate: 96 Well (12 x 8 Well Strips)
  • Testosterone Standard: 6 x 500 uL
  • Antibody: 1 x 6 mL
  • HRP-Conjugate: 1 x 6 mL
  • 20X Wash Buffer: 1 x 15 mL
  • Substrate A: 1 x 7 mL
  • Substrate B: 1 x 7 mL
  • Stop Solution: 1 x 7 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell culture supernates and Tissue homogenates
• Sensitivity: 0.06 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat
  testosterone. No significant cross-reactivity or interference between rat
  testosterone and analogues was observed.
• Publications:

  Hypothyroidism: morphological and metabolic changes in the testis of adult albino rat and the amelioration by alpha lipoic acid. Folia Morphol (Warsz). NULL, NULL (2020). 32644186

  Physiological Roles of Red Carrot Methanolic Extract and Vitamin E to Abrogate Cadmium-Induced Oxidative Challenge and Apoptosis in Rat Testes: Involvement of the Bax/Bcl-2 Ratio. Antioxidants (Basel). 10 (2021). 34829524

• Assay Info: Assay Methodology: Quantitative Competitive ELISA
• Description:
  View product citations for Testosterone ELISA Kit (Rat) (OKCA00179) on CiteAb

Target Info

• Gene Symbol: Testosterone
• Alias Symbols: Testosterone, Testoderm, Sustanon, Testosteron, Androderm, Mertestate
• Protein Name: Testosterone
• Description of Target: Testosterone is a steroid hormone from the androgen group and is found in mammals, reptiles, birds, and other vertebrates. In mammals, testosterone is primarily secreted in the testes of males and the ovaries of females, although small amounts are also secreted by the adrenal glands. It is the principal male sex hormone and an anabolic steroid.