Catalog No: OKCA00142
Size:96 Wells
Price: $550.00
SKU
OKCA00142
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKCA00142
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration< 4 Hours
ELISA Kit PrincipleAviva Systems Biology Testosterone ELISA Kit (Mouse) (OKCA00142) is based on a competitive enzyme immuno assay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for Testosterone and Horseradish Peroxidase (HRP) conjugated Testosterone. The competitive inhibition reaction is launched between with HRP labeled Testosterone and unlabeled Testosterone with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Testosterone in the sample. The color development is stopped and the intensity of the color is measured.
ELISA Kit Range1 - 15 ng/mL
ELISA Kit Reproducibility
Mean Intra-Assay Precision %CVMean Inter-Assay Precision %CV
Sample123123
n202020202020
Mean (ng/mL)1.6181.6051.6121.6171.6291.591
SD0.0410.0470.0410.0460.0520.029
CV%6.677.676.7037.5238.5194.733
ELISA Kit Component
ComponentAmount
Anti-Testosterone Microplate96 Well (12 x 8 Well Strips)
Testosterone Standard6 x 500 uL
Antibody 1 x 6 mL
HRP-Conjugate 1 x 6 mL
20X Wash Buffer1 x 15 mL
Substrate A1 x 7 mL
Substrate B1 x 7 mL
Stop Solution1 x 7 mL
Additional Information

CAS#: 58-22-0

Molecular Formula: C19H28O2

Molecular Weight: 288.42

Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates
Sensitivity< 0.05 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse
testosterone. No significant cross-reactivity or interference between mouse
testosterone and analogues was observed.
Assay InfoAssay Methodology: Quantitative Competitive Immunoassay
Description
Gene SymbolTestosterone
Alias SymbolsTestosterone, Testoderm, Sustanon, Testosteron, Androderm, Mertestate
Protein NameTestosterone
Description of TargetTestosterone is a steroid hormone from the androgen group and is found in mammals, reptiles, birds, and other vertebrates. In mammals, testosterone is primarily secreted in the testes of males and the ovaries of females, although small amounts are also secreted by the adrenal glands. It is the principal male sex hormone and an anabolic steroid.
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