TCF7L2 ELISA Kit (Human) (OKCD09429)

Catalog No: OKCD09429

Size:96WELLS

TCF7L2 ELISA Kit (Human) (OKCD09429) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcription Factor 7 Like Protein 2 (TCF7L2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transcription Factor 7 Like Protein 2 (TCF7L2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transcription Factor 7 Like Protein 2 (TCF7L2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transcription Factor 7 Like Protein 2 (TCF7L2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transcription Factor 7 Like Protein 2 (TCF7L2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transcription Factor 7 Like Protein 2 (TCF7L2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • TCF7L2 Microplate: 96 Wells (12 x 8 Well strips)
  • TCF7L2 Lyophilized Standard: 2
  • 100X TCF7L2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.054ng/mL

Target Info

• Gene Symbol: TCF7L2
• Gene Full Name: transcription factor 7 like 2
• Alias Symbols: HMG box transcription factor 4;hTCF-4;T-cell factor 4;T-cell-specific transcription factor 4;TCF4;TCF-4;transcription factor 7-like 2;transcription factor 7-like 2 (T-cell specific, HMG-box).
• NCBI Gene Id: 6934
• Protein Name: Transcription factor 7-like 2
• Description of Target: Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.
• Uniprot ID: Q9NQB0
• Protein Accession #: NP_001139746.1
• Nucleotide Accession #: NM_001146274.1